I-RJMPDRN®I-REC imele intuthuko ebalulekile ezithakweni zezimonyo ezisekelwe ku-nucleic acid, inikeza i-recombinant salmon PDRN eyenziwe nge-biotechnology. I-PDRN yendabuko ikhishwa kakhulu ku-salmon, inqubo evinjelwa yizindleko eziphakeme, ukuguquguquka kwe-batch-to-batch, kanye nobumsulwa obulinganiselwe. Ngaphezu kwalokho, ukuthembela ezinsizeni zemvelo kudala ukukhathazeka kokusimama kwemvelo futhi kunciphisa ukuguquguquka ukuze kuhlangatshezwane nesidingo semakethe esikhulayo.
I-RJMPDRN®I-REC ibhekana nalezi zinselele ngokusebenzisa izinhlobo zamagciwane eziklanywe ngobunjiniyela ukuze iphindaphinde izingcezu ze-PDRN eziqondiwe, ivumela ukwenziwa okulawulwayo ngenkathi igcina ikhwalithi ephindaphindwayo futhi inciphisa umthelela wemvelo.
Le ndlela yokuhlanganisa kabusha ivumela ukwakheka okunembile kokulandelana kokusebenza, okuholela emikhiqizweni ye-nucleic acid eyenzelwe imiphumela ethile esebenzayo. Isisindo sama-molecule kanye nokuvumelana kwesakhiwo kwezingcezu kulawulwa ngokuqinile, okuthuthukisa kokubili ukufana kanye nokungena kwesikhumba. Njengesithako esingenasilwane, i-RJMPDRN®I-REC ihambisana nezindinganiso zomthetho zomhlaba wonke, yandisa ukwamukelwa kwemakethe ezindaweni ezibucayi. Inqubo yokukhiqiza ilandela izindinganiso zekhwalithi eziqinile, isebenzisa izindlela zokubilisa ezikhula ngokushesha kanye nokuhlanza eziletha ikhwalithi eqhubekayo, ubumsulwa obuphezulu, kanye nokunikezwa okuthembekile—okubhekana nezindleko, uchungechunge lokuhlinzeka, kanye nezinselele zemvelo zokukhishwa okuvamile.
Ngokwemvelo, i-RJMPDRN®I-REC iyimpuphu emhlophe, encibilika emanzini eyakhiwe yi-DNA ene-RNA encane, etholakala ku-salmon PDRN sequences, futhi ibonisa ububanzi be-pH obungu-5.0–9.0. Ihlukaniswa njengesithako sezinga lobuhle esifanele ukusetshenziswa kuma-emulsion aphezulu, okhilimu, ama-eye patches, ama-masks, kanye nezinye izindlela zokunakekelwa kwesikhumba zekhwalithi ephezulu. Izifundo ze-in vitro zibonise ukuphepha kwayo kanye nokusebenza kwayo ekugxilweni okungu-100–200 μg/mL, okusekela ukwanda kwamaseli kanye nomsebenzi wokulwa nokuvuvukala ngaphandle kwe-cytotoxicity.
Izifundo zokusebenza kahle ziqokomisa ukusebenza okuphezulu kwe-RJMPDRN®I-REC. Ithuthukisa kakhulu ukufuduka kwe-fibroblast, ifinyelela izinga lokusabalala elingu-131% emahoreni angu-41 uma kuqhathaniswa nezilawuli. Ngokuphathelene nokuhlanganiswa kwe-collagen, i-RJMPDRN®I-REC ikhuthaza i-collagen yohlobo I lomuntu ngezikhathi eziyi-1.5 kanye ne-collagen yohlobo lwesithathu ngezikhathi eziyi-1.1 uma kuqhathaniswa nezilawuli, idlula i-PDRN evamile etholakala ku-salmon. Ngaphezu kwalokho, ivimbela kakhulu izinto ezibangela ukuvuvukala njenge-TNF-α kanye ne-IL-6. Uma ihlanganiswa ne-sodium hyaluronate, , RJMPDRN®I-REC ikhombisa imiphumela yokusebenzisana, okwandisa ukufuduka kwamaseli, okubonisa amandla amakhulu okubambisana ekunakekelweni kwesikhumba okuvuselelayo nokulwa nokuguga.
Ngamafuphi, i-RJMPDRN®I-REC imele intuthuko kwezobuchwepheshe kusukela ekukhishweni kwendabuko kuya ekuhlanganisweni kwe-biotechnology, ihlinzeka ngendlela ephindaphindwayo, emsulwa kakhulu, futhi eqhubekayo yokwenza izinto zokunakekelwa kwesikhumba ezisezingeni eliphezulu. Ukusebenza kwayo okubonakalayo kwe-bioactive, iphrofayili yokuphepha, kanye nokukhula kwayo kuyibeka njengesithako esibalulekile semikhiqizo yezimonyo ehlose ukulwa nokuguga, ukulungiswa kwesikhumba, kanye nempilo yesikhumba iyonke, okuhambisana ngokugcwele nesidingo esishintshashintshayo sezithako zezimonyo ezisimeme neziqinisekisiwe ngokwesayensi.
Chofoza lapha ukuze ufunde kabanzi ngalo mkhiqizo.
Isikhathi sokuthunyelwe: Agasti-28-2025