Sekungamashumi eminyaka, i-PDRN ithembele ekukhishweni kwamaseli okuzala e-salmon. Le ndlela yendabuko ivinjelwe ukuguquguquka kokutholakala kwezinhlanzi, ukulandelana kwe-DNA okungahleliwe, kanye nezinselele ekulawuleni ubumsulwa—okwenza kube nzima ukuqinisekisa ukuvumelana kwesikhathi eside, ukuguquguquka, kanye nokuhambisana nemithetho.
OkwethuI-Recombinant PDRNyathuthukiswa ukuze inqobe le mikhawulo yesakhiwo ngokusebenzisa ubuchwepheshe obuthuthukisiwe be-bioengineering.
Ayinazo izinsiza zezilwane, eyakhelwe phezu kokwenziwa kwezinto eziphilayo okulawulwayo
Kusetshenziswa i-E. coli DH5α njengeplatifomu yokukhiqiza yezinto eziphilayo, ukulandelana kwe-PDRN ethile kwethulwa ngama-recombinant vectors futhi kuphindaphindwe ngempumelelo ngokubiliswa kwamagciwane.
Le ndlela iqeda ukuncika ezintweni ezitholakala ezinhlanzini, ibhekana nokungazinzi kokuhlinzekwa kanye nokukhathazeka ngokuphepha kwezilwane emthonjeni, ngenkathi ihambisana nezindinganiso zomthetho eziqinile kakhulu ezimakethe ze-EU, e-US, kanye nezomhlaba wonke.
Ngesikhathi esifanayo, umkhiqizo uhlalaOkusekelwe ku-DNA futhi okwenziwe ngokwemvelo, okwenza kubeokunye okungeyona i-vegan, okungeyona isilwane, kodwa okuyiqiniso ngokwezinto eziphilayokuya ku-PDRN yendabuko etholakala ku-salmon.
Ukulandelana okuklanywe kahle, hhayi ukukhipha okungahleliwe
Ngokungafani ne-PDRN evamile etholakala ngokukhipha okungakhethi, ubuchwepheshe be-recombinant buvumelaukulawula okugcwele ukulandelana kwe-DNA kanye nobude bezingcezu.
Uchungechunge olufushane lungaklanywa ukuze kusetshenziswe izinhlelo zokulwa nokuvuvukala
Uchungechunge oluphakathi kuya kolude lungenziwa ngendlela yokuthi lusekele ukuvuselelwa kwe-collagen kanye nokulungiswa kwesikhumba
Lolu shintsho—kusukela ekukhishweni okungahleliwe kuya ekuhlanganisweni kwezinto eziphilayo okuqondiwe—luvula amathuba amasha okuthuthukiswa okuqhutshwa umsebenzi kanye nokwakheka okwenziwe ngezifiso.
Ukukhula kanye nokuphindaphinda kwebanga lezimboni
Ngokuhlanganisa ukuguqulwa kokushisa okuthuthukisiwe kanye nokulungiswa kwamaseli okusebenza kahle kakhulu, ukumuncwa kwe-plasmid kanye nesivuno sokukhiqiza kuthuthuka kakhulu.
Kuhlanganiswe nokucheba umzimba ngezinyathelo eziningi kanye nokuhlanzwa kwe-chromatographic okuhlelekile, inqubo iyaqhubeka nokufezaubumsulwa bezinga lezokwelapha (≥99.5%).
Imingcele yokubilisa ejwayelekile iqinisekisa ukwanda okubushelelezi kusukela ekukhiqizweni kokuhlola kuya ekukhiqizweni kwezentengiselwano.
Ukusebenza kahle kuqinisekiswe ngedatha yangaphambi kokwelashwa
Izifundo zangaphambi kokwelashwa zibonisa ukuthi i-PDRN ephinda ihlanganiswe ilethaukukhuthazwa okuphezulu kokwenziwa kwe-collagen yohlobo lwe-I yomuntuuma kuqhathaniswa ne-PDRN kanye ne-DNA-metal complexes ezivamile ezitholakala ku-salmon.
Le miphumela isekela ukusetshenziswa kwayo ekulungiseni isikhumba nasekulweni nokuguga, okunikezaisisombululo sezithako esilandelwa idatha, esiqhutshwa yindlela yokusebenza.
I-Recombinant PDRN ingaphezu nje kokuthatha indawo—iwukuthuthukiswa kobuchwepheshe.
Ngokuhlanganisa ukwakheka kokulandelana okunembile kanye ne-biosynthesis elawulwayo, ubuchwepheshe obuhlanganisa kabusha bukhulisa ukusebenza kwe-PDRN ngenkathi buhlinzekaokunye okuzinzile, okungenamsoco, kanye nokwemveloku-PDRN etholakala ezilwaneni—ukubeka izinga elisha lezithako zokuvuselela isikhumba zesizukulwane esilandelayo.
Isikhathi sokuthunyelwe: Disemba 31-2025